Rhamnolipid Biosurfactants—Possible Natural Anticancer Agents and Autophagy Inhibitors

Background/Aim: A number of biologically active substances were proved as an alternative to conventional anticancer medicines. The aim the study is in vitro investigation activity mono- and di-Rhamnolipids (RL-1 RL-2) against human breast cancer. Additionally, combination with Cisplatin was analyzed. Materials Methods: Breast cell lines (MCF-10A, MCF-7 MDA-MB-231) treated RLs Cisplatin. viability analyzed using MTT assay, autophagy performed via acridine orange staining. Results: In contrast healthy cells, both tested cancer exhibited sensitivity treatment. This effect accompanied by influence on autophagy-related acidic formation process. Only for triple-negative line (MDA-MB-231) synergistic combined treatment (10 µM 1 µg/mL observed. Conclusion: Based studies reorganization membrane models presence RL data about a higher amount lipid rafts membranes than non-tumorigenic, we suggest possible mechanism remodelling endosomes. Shortly, order have effect, it necessary andRL-2 RL2 molecule inducingpositive curvature.